Laser-induced fluorescence imaging of subsurface tissue structures with a volume holographic spatial-spectral imaging system.

نویسندگان

  • Yuan Luo
  • Paul J Gelsinger-Austin
  • Jonathan M Watson
  • George Barbastathis
  • Jennifer K Barton
  • Raymond K Kostuk
چکیده

A three-dimensional imaging system incorporating multiplexed holographic gratings to visualize fluorescence tissue structures is presented. Holographic gratings formed in volume recording materials such as a phenanthrenquinone poly(methyl methacrylate) photopolymer have narrowband angular and spectral transmittance filtering properties that enable obtaining spatial-spectral information within an object. We demonstrate this imaging system's ability to obtain multiple depth-resolved fluorescence images simultaneously.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Spectrum resolved fluorescence imaging in multi-focal volume holographic microscopy

A real-time three dimensional (3D) fluorescence imaging system incorporating wavelength-coded and multiplexed holographic gratings is presented. Holographic gratings formed in thick Phenanthrenquinone(PQ-) Doped Poly (methyl methacrylate) (PMMA) have narrowband spectral-spatial transmittance filtering properties to generate wavelengthspectrum selective multi-focal planes within a biological obj...

متن کامل

Spectral Separation of Quantum Dots within Tissue Equivalent Phantom Using Linear Unmixing Methods in Multispectral Fluorescence Reflectance Imaging

Introduction Non-invasive Fluorescent Reflectance Imaging (FRI) is used for accessing physiological and molecular processes in biological media. The aim of this article is to separate the overlapping emission spectra of quantum dots within tissue-equivalent phantom using SVD, Jacobi SVD, and NMF methods in the FRI mode. Materials and Methods In this article, a tissue-like phantom and an optical...

متن کامل

Simultaneous multiplane imaging of human ovarian cancer by volume holographic imaging.

Ovarian cancer is the most deadly gynecologic cancer, a fact which is attributable to poor early detection and survival once the disease has reached advanced stages. Intraoperative laparoscopic volume holographic imaging has the potential to provide simultaneous visualization of surface and subsurface structures in ovarian tissues for improved assessment of developing ovarian cancer. In this ex...

متن کامل

Toward real-time virtual biopsy of oral lesions using confocal laser endomicroscopy interfaced with embedded computing.

Oral lesions are conventionally diagnosed using white light endoscopy and histopathology. This can pose a challenge because the lesions may be difficult to visualise under white light illumination. Confocal laser endomicroscopy can be used for confocal fluorescence imaging of surface and subsurface cellular and tissue structures. To move toward real-time "virtual" biopsy of oral lesions, we int...

متن کامل

Volume holographic spectral imaging

We report on a volume holographic imaging spectrometer (VHIS) system which allows retrieval of a scene's twodimensional spatial information as well as its spectral information. This is performed using a transmission volume hologram and a simple rotary scanning mechanism. The system has the advantages of high spectral and spatial resolutions and the potential of single-shot, four-dimensional (3D...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Optics letters

دوره 33 18  شماره 

صفحات  -

تاریخ انتشار 2008